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1. With glass microelectrode technique specific for calcium ion, measurements were made for serum ionized calcium. The calibration curve was linear for pCa(2-4) and the tests were reproducible. 2. For five persons each with normal health and with uremia, serum creatinine above 10㎎/㎗, ionized calcium, total calcium, total protein, albumin, and inorganic phosphate were measured in the sera. The results are as following: 1) There were significant differences in serum phosphate and ionized calcium levels between healthy group and uremic patients. The differences were not significant in total calcium, total protein, and albumin contents. 2) The values of ionized calcium, total calcium, total protein and albumin were scattered, but individually some with uremia had lower values. 3. Preliminary tests for ionized calcium assay gave the experience that the ways of collecting and storing samples would alter the data significantly. 4. It is, therefore, felt that further studies on simplification of the sample handing before the measurement of ionized calcium is desirable.
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